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Kima Pump

The Kima^TM Pump is a microfluidic pump designed to aid cell culture (e.g. endothelial cells) under physiological conditions (shear flow) in various biochips and flow chambers, including Cellix's Vena8 Endothelial+ biochips where it is possible to culture 8 cell monolayers simultaneously over 24-48 hours. The Kima^TM pump is controlled by the iKima^TM Application for use with the iPod Touch and iPhone. The Kima^TM pump is placed inside a standard incubator while the iPod Touch or iPhone, as it's controller, sits on the Universal dock outside the incubator. The Kima^TM pump connects easily to Cellix's Vena8 Endothelial+ biochip and other manufacturer's flow chambers via fully autoclaveable tubing and delivers pulses of fresh media to cells seeded inside the microchannels. The iKima^TM App will shortly be available for download from Apple's iTunes Store.

Kima^TM Pump with iPod Touch and Controller Kima Pump connected via tubing to Vena8 Endothelial+ biochip		iKima^TM App

HUVEC primary cells seeded in Vena8 Endothelial+ biochip: 1.5hrs post-incubation	HUVEC primary cells seeded in Vena8 Endothelial+ biochip: 72hrs post-perfusion with Kima pump

iKima^TM Application: How does it work?

iKima^TMis an Apple Application for iPod Touch or iPhone which controls the Kima^TM pump. There are three main steps for the application: set-up; wash-out and flow assay as illustrated below.

1. Set-up	2. Wash-out	3. Flow assay
Select iKima App on iPod Touch or iPhone. Connect iPod Touch to Kima controller: Press "Connect" button.	To wash-out the pump: Press "Wash". To stop washing: Press "Stop".	To start perfusion in the biochip or flow chamber: Press "Perfuse" and select "Perfusion Time" (time for which media will continue perfusing) and "Pause Time" (interval between perfusion times).