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Harvard Medical School publish paper in Nature Immunology using Cellix's platform |
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Inositol hexakisphosphate kinase 1 regulates neutrophil function in innate immunity by inhibiting phosphatidylinositol-(3,4,5)-triphosphate signaling
Amit Prasad, Yonghui Jia, Anutosh Chakraborty, Yitang Li, Supriya K Jain, Jia Zhong, Saurabh Ghosh Roy, Fabien Loison, Subhanjan Mondal, Jiro Sakai, Catlyn Blanchard, Solomon H Snyder and Hongbo R Luo
It was shown that an InsP6K1 does not regulate neutrophil trafficking and survival. During the investigation of peritonitis models described in the paper and supplemental information; researchers observed attenuated peritoneal accumulation of neutrophils in Ip6k1-/- mice where one of the possible causes indicated may be the alternation of neutrophil trafficking from the circulation to the inflamed peritoneal cavity. However, using Cellix's Mirus Nanopump and Vena8 biochips; neutrophil adhesion assays to fibronectin under shear flow were performed where an increase in PtdIns(3,4,5)P3 signaling achieved by disruption of InsP6K1 failed to further augment cell adhesion, directionality and migration speed in mouse neutrophils (see Supplementary Fig. 8 and Supplementary Movies 3-4).
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